Yeah, they're female. As the bottom most cards on the Tree of Life, the "Thrones of the Aces," sharing a place with all the Tens, they are the ones who receive all of creation. They're the Courts closest to us, normal people who use whatever the world has to offer and creating new things, thus being a starting point for the new cycle. Symbolically female attributes.
Also she's the bride of the Holy Guardian Angel. It don't remember where I read that in the Goldman Dawn all aspirants were symbolically female.
Plus, since we're talking about Waite and his Christian influences, the whole cup and fish thing could be an allusion to some Waite thing like the Grail and the Jesus fish or something like that.